A Few Immobilized Thrombins Are Sufficient for Platelet Spreading

A few immobilized thrombins are sufficient for platelet spreading.

Eukaryotic cells respond to signaling molecules with picomolar to nanomolar sensitivities. However, molar concentrations give no suggestion of the sufficient number of molecules per cell and are confusing when referring to physiological situations in which signaling molecules act in an immobilized state. Here, we studied platelet adhesion by thrombin, a key step in normal hemostasis and patholo...

Calcium-and integrin-binding protein regulates focal adhesion kinase activity during platelet spreading on immobilized fibrinogen.

Platelet spreading on the subendothelium in response to vascular injury is fundamental to the regulation of physiologic hemostasis. Previously, we have shown that, when bound to glycoprotein IIb (GPIIb), calcium- and integrin-binding protein (CIB) regulates platelet spreading on immobilized fibrinogen (Fg). In this study, we investigated the signaling events that occur downstream of CIB in the ...

On Sufficient Conditions for a Linearly Determinate Spreading Speed

It is shown how to construct criteria of the form f(u) ≤ f ′(0)K(u) which guarantee that the spreading speed c∗ of a reaction-diffusion equation with the reaction term f(u) is linearly determinate in the sense that c∗ = 2 p f ′(0). Some of these criteria improve the classical condition f(u) ≤ f ′(0)u, and permit the presence of sharp Allee effects. Inequalities which guarantee the failure of li...

Human Thrombins

Human o-thrombin, the thromboplastin activation product of prothrombin with high clotting and e&erase activity, was produced from Cohn Fraction III paste. The procedure started with 0.4 to 3.2 kg of frozen paste and was completed in 2 or 3 days. Some 23 g of thrombin were recorded for 65 quantitated preparations made from 11 lots of Fraction III paste. These preparations were obtained at protei...

Border Disease Control Checkpoints: Are They Sufficient for Disease Control? The Present Situation in Thailand